Enhancing Saccharide Extraction from Konjac by Microwave-assisted Acid Hydrolysis
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Abstract
This article presents a study on the use of microwave-assisted acid hydrolysis to produce glucomannan oligosaccharides from konjac powder. Acid hydrolysis is a well-established method for the depolymerization of polysaccharides. This study aims to optimize the conditions of acid hydrolysis using microwave radiation to achieve high yields and specific saccharide sizes. Konjac powder was hydrolyzed using hydrochloric acid in a microwave digestion instrument. The optimal conditions for hydrolysis were determined based on previous research, and the resulting soluble oligosaccharides were purified by ethanol precipitation. The carbohydrate content and size distribution of the hydrolyzed products were analyzed by using phenol-sulfuric acid assay and size exclusion chromatography, respectively. This study showed that microwave-assisted acid hydrolysis is a promising method for the preparation of glucomannan oligosaccharides from konjac powder, offering advantages such as shorter reaction time and lower acid catalyst requirement. The results contribute to the understanding of konjac powder hydrolysis and provide insights for the development of efficient and sustainable production processes for glucomannan oligosaccharides.
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References
Bui CV, Siriwatwechakul W, Tiyabhorn W, Wattanasiritham T, Limpraditthanont N, Boonyarattanakalin S. Conversion of konjac powder into glucomannanoligosaccharides, mannose, and glucose by hydrolysis facilitated by microwave heating and HCl catalyst. J. Ind. Technol. 2016;12(2):45-61.
You S, Ding J, Dai Y, Xing R, Qi W, Wang M, et al. A simply enzymatic hydrolysis pretreatment for beta-mannanase production from konjac powder. Bioresour. Technol. 2017.
Jian W, Sun Y, Huang H, Yang Y, Peng S, Xiong B, et al. Study on preparation and separation of Konjac oligosaccharides. Carbohydr. Polym. 2013;92(2):1218-24.
Katsuraya K, Okuyama K, Hatanaka K, Oshima R, Sato T, Matsuzaki K. Constitution of konjac glucomannan: chemical analysis and 13C NMR spectroscopy. Carbohydr. Polym. 2003;53(2):183-9.
Warrand J, Janssen H-G. Controlled production of oligosaccharides from amylose by acid-hydrolysis under microwave treatment: Comparison with conventional heating. Carbohydr. Polym. 2007;69(2):353-62.
de Moura FA, Macagnan FT, da Silva LP. Oligosaccharide production by hydrolysis of polysaccharides: A review. Int. J. Food Sci. Technol. 2015;50(2):275-81.
Ávila-Fernández Á, Galicia-Lagunas N, Rodríguez-Alegría ME, Olvera C, LópezMunguía A. Production of functional oligosaccharides through limited acid hydrolysis of agave fructans. Food Chem. 2011;129(2):380-6.
Hu K, Liu Q, Wang S, Ding K. New oligosaccharides prepared by acid hydrolysis of the polysaccharides from Nerium indicum Mill and their anti-angiogenesis activities. Carbohydr. Res. 2009;344(2):198-203.
Du B, Song Y, Hu X, Liao X, Ni Y, Li Q. Oligosaccharides prepared by acid hydrolysis of polysaccharides from pumpkin (Cucurbita moschata) pulp and their prebiotic activities. Int. J. Food Sci. Technol. 2011;46(5):982-7.
Burana-osot J, Soonthornchareonnon N, Hosoyama S, Linhardt RJ, Toida T. Partial depolymerization of pectin by a photochemical reaction. Carbohydr. Res. 2010;345(9):1205-10.
Song Q, Wu L, Li S, Zhao G, Cheng Y, Zhou Y. Aggregation of konjac glucomannan by ethanol under low-alkali treatment. Food Chem.: X 2022;15:100407.