The Effect of pH Immobilization of Antibody-B on the GPTMS Functionalized Filter Paper for Detect Antigen B on the Red Blood Cell Surface
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Abstract
The filter paper-based analytical platform has been interested in recent years due to its availability and inexpensiveness. This work focused on the silane functionalized filter paper which was developed for immobilization of antibody probe, anti-B, for detecting their specific antigen, antigen-B, on red blood cells surface. 3-Glycidoxypropyltrimethoxysilane (GPTMS) was hydrolyzed under a basic solution to conduct anti-B immobilized onto the paper via covalent bonding. The concentration of GPTMS was compared between 0.5% and 5% v/v to obtain the strong signal of red blood cells adhering to the surface. The functionalized surface was characterized with the FTIR technique. Then the pH of the buffer solution was varied to find the suitable pH for linking the antibody onto the silanized filter paper. The results show that using a high concentration of GPTMS can enhance the antibody and antigen interaction. At strong pH (pH 9) solution, the opening epoxide ring can occur, and antibodies were linked onto the surface via this reaction. The interaction of antibody-B and antigen on red blood cells was measured by the mean intensity of the color present on the paper and confirmed red blood cells adhering by scanning electron microscope. The GPTMS functionalized filter paper can enhance the bioactivity of specific interaction of antibody-B and red blood cell-B and the uniformity of the reaction.
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